Three Faculty of Agriculture Master’s students showcased their preliminary research results at the Postgraduate Research Open Day held by the Agricultural Research Council (ARC) Tropical and subtropical Crops (TSC) in Nelspruit.
Second year Master’s students, Nicholus Mnyambo and Moses Timana, and first year Master’s student, Lerato Kgotse, also presented at the 23rd Nematological Society of Southern Africa (NSSA) in Tulbagh, Western Cape. NSSA is a prestigious conference of nematologists from Southern Africa, which attracts nematology experts from around the world.
“As young scientists, the conference provided us with the opportunity to network with researchers and students from various research institutions,” said Timana.
Mnyambo added that the opportunity has increased his networking skills, improved his communication and presentation abilities, and has kept him up to date with the latest information in his research area.
“One of the biggest benefits was getting to know other people in my field, networking, sharing ideas, and exchanging contacts just in case need I need advice from an expert in another field, and specifically from people at my level of study,” he said.
Kgotse said that since the conference she has become more interested in research than before. "I am excited about continuing my career in the research field. Presenting my results to other researchers gave me an even better understanding of my own research, as well as the confidence to conduct more studies to share with others."
Lerato Kgotse: MSc Student in Agriculture (First year)
Research Topic: Rhizobium leguminosarun and Meloidogyne enterolobii effects on growth and nodulation of retained cowpea cultivars.
“My research seeks to characterize the relationship between meloidogyne enterolobii a recently identified economically important root-knot nematode on soybean and rhizobium leguminosarum on the growth of local cowpea varieties for potential use in the management of nematode in communal farming areas.
Understanding the relationship between the two species will help with knowing if the bacteria (Rhizobium leguminosarum) is able to suppress or control the nematode population and reduce the damage on the plant or the nematodes suppress or outcompetes the bacteria as they occupy the same space on the plant.
During my honours degree I had a nematology research project, and when doing data collection, I discovered the presence of bacterial nodules on the roots of the nematode inoculated plants and that got interested in the type of relationship these two microorganisms have, and their effect on the plant. That’s when decided I should take this to my master’s level, I also figured that, maybe the bacteria could somehow be used to control nematode.
The relationship between the two microorganisms were observed to vary with cultivar. Time of bacterial inoculation in relation to the nematode did not differ but cultivars differed in their response to the nematode species. Meloidogyne enterolobiihad differing effects on number of total bacterial nodules and number of active nodules formed on roots of different cultivars. Of the six cultivars “Cv17A” had significantly the highest number of nematode J2 and eggs in the roots while “Cv17D” had significantly the lowest number of J2 and eggs in roots.
The treatment that had the highest number of nematode J2 and eggs in the roots was nematode inoculation first while the lowest was rhizobia inoculation first. The relationship between nematodes and nitrogen fixing bacterial is more complex than previously reported, with this relationship probably varying with nematode species, plant species and cultivar within each plant species.
In the current study nematodes had a direct effect on the cowpea nodulation and proportion of active versus inactive nodule numbers irrespective of nematode inoculation time relative to the time at which nitrogen fixing bacteria inoculation was done.”
Nicholus Mnyambo: MSc in Agriculture (Second Year)
Research Topic:: Resistance status of Bambara groundnuts to root-knot nematodes and nematodes management using plant extracts.
“My research focuses on the use of a traditional plant, Bambara groundnut also known as tindlubu in Siswati. Bambara groundnut is a traditional underutilized crop which is important as a food source, particularly for most rural communities where levels of food insecurity are very high. The crop is grown in marginal soils high in nematode infestations known to reduce yield.
Currently, there is limited information on the impact of these nematodes on Bambara groundnuts. This research will provide communal farmers with information on the potential impact of nematodes on the production of the crop and give an alternative approach in controlling the nematodes population using cheaper reliable methods. In commercial agriculture, these nematodes are controlled through the use of chemicals.
These chemicals are expensive for smallholder farmers. The study seeks to look into an environmentally friendly, human-friendly, cost-effective method that can work in controlling the nematodes. The study used plant extract from an indigenous plant called Maerua angolensisto create a nematode control method.
The study seeks to achieve a natural human and environmentally friendly nematodes control method that is affordable to the people in rural areas where this crop is produced. Achieving this will allow us to control nematodes and promote Bambara groundnut. In Africa, Bambara groundnut is ranked third as a protein-rich crop. Bambara groundnut has a potential in addressing malnutrition and food insecurity in rural areas.
Growing up in a family that is agriculture-orientated made me fall in love with agriculture. Moreover, there is a huge gap between production from commercial agriculture and production from substance farming. This is due to the resources available in commercial agriculture, which are absent in rural areas. People from rural communities don’t have enough capital to purchase these chemicals that assist in improving yield.
Also, there is an increase in the general awareness of the harmful effect that these chemicals have on the environment and human health, which resulted in some being withdrawn or restricted from the market. My dream is to promote the traditional crops and try to assist in addressing issues caused by the growing human populations such as high food demand.
The research will provide an alternative cheap method that can be adopted by farmers and control nematodes while simultaneously improving yields.”
Moses Timana: MSc in Agriculture (Second year)
Research Topic: Host response of local cassava varieties to root-knot nematode infection and their management with plant extracts.
"In my research we were looking at the susceptibility and sensitivity of Cassava to a plant parasitic nematode species called Meloidogyne incognita. It was an interesting experience because there were a number of things that were impromptu, this includes observing the cassava Mosaic virus which is a major problem in cassava production and also trying to find ways to deal with it in such a way that it does not have an effect of our research results.
Another challenge we came across was the plant-sprouting percentage being poor and we then investigated a number of possible factors that may have been the cause, factors such as the environment our plants were grown in, the size and position of the cuttings, the growing media, and also priming of the cuttings.
From the preliminary results we can tell that the plant is tolerant, but we still have a second trial at the farm just to validate our findings, provided the story tallies. We were also looking at the impact of plant extracts of Maerua angolensi, which is a medicinal plant, on the management of M. incognitabecause studies have shown that this plant can suppress nematode populations in tomatoes, so the plant extracts had similar effects with our positive control. Another interesting observation was improved plant growth in plants treated with this, so we suspect that these extracts may have improved the rhizosphere, so we need to investigate this in future.
We also found that the environment this is grown in affects sprouting and root mass, while using rooting hormone and peat as a growing media also improved sprouting.”
@ Story by Lisa Thabethe. Pictures Supplied.